Instrumental Analytical Chemistry Lab

The analytical instrumental chemistry research team is skilled in the development of new methodologies and instrumentation for the determination and characterization of trace and ultra-trace elements and compounds in biological, clinical, environmental samples by atomic spectrometry, electrochemical, chromatographic and hyphenated techniques (liquid chromatography and gas chromatography coupled to atomic absorption and fluorescence spectrometry, mass spectrometry).They have a long term experience in spectroscopic techniques (infrared spectroscopy, light scattering, molecular fluorescence and UV-vis spectroscopy). They have been pioneers in the development and implementation of on-line instrumental devices for surface tension and viscosity measurements for the characterization of surface activity of polymers and proteins.

Research topics

  • Process analytical chemistry
  • Environmental monitoring
  • Protein Chemistry
  • Clinical Chemistry
  • Cultural Heritage
  • Trace and ultra-trace metal determination and speciation (mercury, selenium, arsenic…) in raw matrices
  • Mechanisms of hydrolysis of borane complexes and hydrogen transfer
  • Novel derivatization techniques for CG-MS

Expertise

  • Spectrochemical methods: atomic spectroscopy, UV-Vis, FT-IR, Raman
  • Chromatographic techniques: GC, HPLC (SEC, GPC, RPC, HIC, IC), GC-MS
  • Electrochemistry
  • Chemometrics
  • Microwave technology
  • Thermoanalytical techniques (DSC, dynamic mechanical analyser, TGA)
  • Hyphenated techniques:
    • Chemical Vapor Generation (CVG) /Atomic Spectrometry- Mass Spectrometry
    • Photochemical Generation / Atomic Spectrometry- Mass Spectrometry
    • UV / Microwave on line sample oxidation
    • GC / HPLC / Spectrometry
    • HPLC/ICP-MS
    • HPLC / Dynamic Surface Tension Detector

Instruments

  • MW FKB Milestone StartD Digestor with temperature control
  • Eppendorf 5804R Centrifuge and lyophilyzer
  • FTIR Perkin Elmer Spectrum 100 spectrophotometer equipped with ATRU
  • UV-Visible Lambda 25 Perkin Elmer spectrophotometer
  • LS45 Perkin Elmer spectrofluorimeter
  • DSC7 Perkin Elmer; DMA 7e Perkin Elmer; TG/DTA 7200 Exstar.
  • 2 HPLC systems ThermoFinnigan with gradient pump (P2000 and P4000), Diode Array UV-Visibile detector (UV6000) and fluorescence detector (FL3000,) and HPLC microbore (Jasco) pumps for on line derivatization.
  • GPC Agilent Technologies 1260 Infinity with Refractive Index detector
  • CG/MS 6850 gas chromatographer coupled with mass detector 5975C MSD (Agilent Technologies, Milan, Italy)
  • Flame Atomic Absorption Spectrometer (AAS)
  • 2 prototype Atomic Fluorescence Spectrometers (AFS) with chemical vapor generation (CVG) with flow injection (FIA) or hyphenation with HPLC.
  • A prototype of a dynamic surface tension detector (DSTD) for the analysis of water soluble surfactants, proteins and polymers using flow injection (FIA) and HPLC techniques
  • ICP Optical Emission Varian 720 ES
  • Direct Mercury Analyzer (DMA 80, Milestone)
  • ICP MS 7700 series Agilent Technologies