Analysis of commercial beverage products by size exclusion chromatography coupled with UV-vis absorbance detection and dynamic surface tension detection

TitleAnalysis of commercial beverage products by size exclusion chromatography coupled with UV-vis absorbance detection and dynamic surface tension detection
Publication TypeJournal Article
Year of Publication2010
AuthorsPierce, KM, Bramanti, E, Onor, M, Spiniello, R, Kangas, A, Skogerboe, KJ, Synovec, RE
ISSN00399140 (ISSN)
KeywordsAbsorbances, Active proteins, animal, Animals, Article, beverage, Beverage products, Beverage samples, Beverages, Body fluids, Bovine serum albumins, Capillarity, Carbonic anhydrases, Cattle, chemistry, Chicken egg white, Chromatographic analysis, Chromatography, Coffee, Commercial standards, Counter anions, Cytochrome C, Detectors, Dynamic surface tension detectors, Dynamic surface tensions, Enzymes, Flow injection analysis, Flow-injection analysis, Food Analysis, Gel, gel chromatography, gel permeation chromatography, human, Human serum albumins, Humans, Instant coffee, instrumentation, Lactoglobulin, Limit of detection, Liquid, Liquid chromatography, Liquids, methodology, Modifying agents, Multidimensional analysis, Organic polymers, polysaccharide, Polysaccharides, protein, Proteins, Quaternary Ammonium Compounds, quaternary ammonium derivative, Rnase A, Selective analysis, Signal detection, Size exclusion chromatography, Solubility, Spectrophotometry, Surface chemistry, Surface pressures, Surface Properties, Surface tension, Surface waves, Tetrabutylammonium, Tetrabutylammonium (TBA), Tetrabutylammonium bromide, time, Time Factors, Ultraviolet, ultraviolet spectrophotometry, UV-vis absorbance, water, Wetting

Multidimensional analysis of instant coffee and barley beverage samples using size exclusion chromatography (SEC) combined with a dynamic surface tension detector (DSTD) and a UV-vis absorbance detector (UV) is reported. A unique finding of this study was the action of the tetrabutylammonium (TBA) cation as a modifying agent (with bromide as the counter anion) that substantially increased the surface pressure signal and sensitivity of many of the proteins in the chromatographically separated samples. The tetrabutylammonium bromide (TBAB) enhancement of the surface pressure signal was further investigated by studying the response of 12 commercial standard proteins ($\alpha$-lactalbumin, $\beta$-lactoglobulin, human serum albumin (HSA), albumin from chicken egg white (OVA), bovine serum albumin (BSA), hemoglobin, $\alpha$-chymotrypsinogen A, cytochrome C, myoglobin, RNase A, carbonic anhydrase, and lysozyme) in buffer performed using flow injection analysis (FIA) coupled with the DSTD with and without various concentrations of TBAB. The FIA-DSTD data show that 1 mM TBAB enhances sensitivity of HSA detection, by lowering the limit of detection (LOD) from 2 mg/mL to 0.1 mg/mL. Similarly, the LOD for BSA was reduced from 1 mg/mL to 0.2 mg/mL. These FIA-DSTD experiments allowed the detection conditions to be optimized for further SEC-UV/DSTD experiments. Thus, the SEC-UV/DSTD system has been optimized and successfully applied to the selective analysis of surface-active protein fractions in a commercial instant coffee sample and in a soluble barley sample. The complementary selectivity of using the DSTD relative to an absorbance detector is also demonstrated. © 2009 Elsevier B.V. All rights reserved.