Reply to comments of Tsikas on "Determination of S-nitrosoglutathione and other nitrosothiols by p-hydroxymercurybenzoate derivatization and reverse phase chromatography coupled with chemical vapor generation atomic fluorescence detection" by Bramanti et

TitleReply to comments of Tsikas on "Determination of S-nitrosoglutathione and other nitrosothiols by p-hydroxymercurybenzoate derivatization and reverse phase chromatography coupled with chemical vapor generation atomic fluorescence detection" by Bramanti et
Publication TypeJournal Article
Year of Publication2009
AuthorsBramanti, E, Angeli, V, Mester, Z, D'Ulivo, A
JournalTalanta
Volume79
Pagination554–555
ISSN00399140 (ISSN)
KeywordsAnalytical methods, S-nitrosothiols
Abstract

Some views are presented on the to comments of Tsikas on determination of S-nitrosoglutathione and other nitrosothiols by p-hydroxymercurybenzoate derivatization and reverse phase chromatography coupled with chemical vapor generation atomic fluorescence detection. It is revealed that RSNO concentration in plasma and its role is being investigated by a team of researchers. The views also reveal that spike recovery measurement has emerged as a useful tool of method evaluation in the absence of certified reference materials, samples tested in an interlaboratory exercise, or consolidated analytical methods and protocols. The specificity of the method depends on the retention time and on the highly selective elemental detection of the mercury tag associated with the thiols.

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-67649608020&partnerID=40&md5=50d9e096462413edb2399e22f17cb77c
DOI10.1016/j.talanta.2009.04.021